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Can digested complementary strand be used as a primer for Sanger sequencing?
Imagine you have a double stranded piece DNA you want to sequence and you know nothing about the sequence. You run a denaturing electrophoresis on it to separate the two strands. You take one of the strands and digest it with endonuclease. You than run gel electrophoresis on the digested strand alongside a DNA ladder, you than select a fragment of desired length and use it as a primer in the Sanger sequencing of the other strand. Is there any reason this wouldn**’**t work? If no, is there a reason why isn**’**t this technique used?
#animals
Imagine you have a double stranded piece DNA you want to sequence and you know nothing about the sequence. You run a denaturing electrophoresis on it to separate the two strands. You take one of the strands and digest it with endonuclease. You than run gel electrophoresis on the digested strand alongside a DNA ladder, you than select a fragment of desired length and use it as a primer in the Sanger sequencing of the other strand. Is there any reason this wouldn**’**t work? If no, is there a reason why isn**’**t this technique used?
#animals
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