Help on FIJI for my Organoids Hi guys, first of all, sorry…
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Help on FIJI for my Organoids
Hi guys, first of all, sorry for my english.
I'm a master student in BioMedicine and I'm attending my year-intership in a cancer-neuroscience lab.
I'm working with cerebellar organoids, and we want to see how the tumor grows in different ways.
Now, the professor asked to me to analyze all our immunofluorescence that we took after the cryostate cut and he told me to quantify all these immunofluorescencefor a poster.
Now, I checked on internet that I have to do on Fini this sequence: threshold->Analyze->analyze particles and after analyzing all the channels I have to calculate the Mitotic Index.
So, the problem arrives now.
When I analyze the particles (the nuclei) the results are different everytime, especially in the DAPI channel.
Sometimes it shows 9000 nuclei and when I re-check it appears something like 230k nuclei and exc...
My Organoids has a dimension of 3mm more or less, it depents how quickly the tumor grows.
Does anyone have an idea of how many nuclei should be present on the slice, or do you know a more accurate way to quantify my immunofluorescence?
Thanks everyone
#animals
source
Hi guys, first of all, sorry for my english.
I'm a master student in BioMedicine and I'm attending my year-intership in a cancer-neuroscience lab.
I'm working with cerebellar organoids, and we want to see how the tumor grows in different ways.
Now, the professor asked to me to analyze all our immunofluorescence that we took after the cryostate cut and he told me to quantify all these immunofluorescencefor a poster.
Now, I checked on internet that I have to do on Fini this sequence: threshold->Analyze->analyze particles and after analyzing all the channels I have to calculate the Mitotic Index.
So, the problem arrives now.
When I analyze the particles (the nuclei) the results are different everytime, especially in the DAPI channel.
Sometimes it shows 9000 nuclei and when I re-check it appears something like 230k nuclei and exc...
My Organoids has a dimension of 3mm more or less, it depents how quickly the tumor grows.
Does anyone have an idea of how many nuclei should be present on the slice, or do you know a more accurate way to quantify my immunofluorescence?
Thanks everyone
#animals
source
| 3,000 mlx | |
| 3 | |
| 0 |