How are whole genome sequences performed? How do we know…
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How are whole genome sequences performed? How do we know the size of it and the position of individual genes?
So don't worry about how complex the answer or indeed the language is. I have a biology education. I just realised I never learned how proper sequencing is performed. Like, WGS. I know that for amplification you use primers to bind to known segments outside of a gene, followed by secondary primers for better quality. Is that what WGS basically is, aside from obviously using NGS techniques?
How do they find the position of a gene? Is a whole genome sequencing not them literally checking the entire genome? What sort of primers do you use for that? Or are primers not crucial anymore, if we can have bases just stick to the readable strand anyway?
#science
So don't worry about how complex the answer or indeed the language is. I have a biology education. I just realised I never learned how proper sequencing is performed. Like, WGS. I know that for amplification you use primers to bind to known segments outside of a gene, followed by secondary primers for better quality. Is that what WGS basically is, aside from obviously using NGS techniques?
How do they find the position of a gene? Is a whole genome sequencing not them literally checking the entire genome? What sort of primers do you use for that? Or are primers not crucial anymore, if we can have bases just stick to the readable strand anyway?
#science
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